Capillary electrophoresis of human follicular fluid
نویسندگان
چکیده
منابع مشابه
Capillary electrophoresis of human follicular fluid.
Some of the major serum proteins that are also found in follicular fluid, including transferrin, alpha-macroglobulin and albumin, are thought to play a role in oocyte maturation. This study set out to identify proteins in human follicular fluid by capillary zone electrophoresis and to investigate their relationship to follicular/oocyte maturity and fertility outcome. 176 individual follicular f...
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A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
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The discovery that antigen}antibody interaction could be produced not only in liquids, but also on gel media, such as agar or agarose gels, with the formation of insoluble immunoprecipitates, opened the door to the development of the gel diffusion techniques for immunoprecipitation analysis. The Rrst of these techniques, known as double diffusion, was introduced by Ouchterlony in 1948. In this ...
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While capillary electrophoresis, or historically related techniques, have been used for over a century, and recognition of the value of this separation methodology has certainly grown rapidly in the past few years, the technique has generally been used by analytical chemists, particularly in Europe and Japan, and small groups of researchers in the United States. Many of the basic instrumentatio...
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Electrophoretic separations carried out in capillary tubes offer the possibilities of rapid and automated analyses of small volumes of complex mixtures with unprecedented resolution and sensitivity. Some emerging developments of this new instrumentation are reviewed.
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ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2009
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2009.09.046